Publications 1998 - 2002

Herpes simplex virus type 1/adeno-associated virus hybrid vectors mediate site-specific integration at the adeno-associated virus preintegration site, AAVS1, on human chromosome 19.Heister T, Heid I, Ackermann M, Fraefel C.
J Virol. 2002 Jul;76(14):7163-73

Quantification of feline herpesvirus 1 DNA in ocular fluid samples of clinically diseased cats by real-time TaqMan PCR.Vögtlin A, Fraefel C, Albini S, Leutenegger CM, Schraner E, Spiess B, Lutz H, Ackermann M.
J Clin Microbiol. 2002 Feb;40(2):519-23

Herpes simplex virus type 1 amplicon vector-mediated gene transfer to muscle.
Wang Y, Mukherjee S, Fraefel C, Breakefield XO, Allen PD.
Hum Gene Ther. 2002 Jan 20;13(2):261-73

Herpes simplex virus/adeno-associated virus hybrid vectors for gene transfer to neurons. Preparation and use.
Costantini L.C., C. Fraefel, X.O. Breakefield and O. Isacson
Methods Mol. Med. 69:461-79 (2002)

HSV-1 amplicon vectors.
Wang S., C. Fraefel and X.O. Breakefield
Methods Enzymol. 346:593-603 (2002)

Transduction of Vero cells and bovine monocytes with a herpes simplex virus-1 based amplicon carrying the gene for the bovine herpesvirus-1 Circ protein.
Schwyzer M., C. Fischer-Bracher, C. Fraefel, T. Bachi, R. Nunez, M. Engels and M. Ackermann
Vet. Microbiol 2002 Apr 22;86(1-2):165-74. doi: 10.1016/s0378-1135(01)00500-4

HSV-1-based amplicon particles are able to transduce cells of feline origin with genes encoding biologically functional feline IL-10 or IL-6.
Vogtlin A., C. Fraefel, R. Kocherhans, C.M. Leutenegger, K. Frei, A. Fontana and M. Ackermann
Vet. Microbiol. 2002 Apr 22;86(1-2):103-13. doi: 10.1016/s0378-1135(01)00495-3

Improved helper virus-free packaging system for HSV amplicon vectors using an ICP27-deleted, oversized HSV-1 DNA in a bacterial artificial chromosome.Saeki Y, Fraefel C, Ichikawa T, Breakefield XO, Chiocca EA.
Mol Ther. 2001 Apr;3(4):591-601

Flow cytometric assessment of transduction efficiency and cytotoxicity of herpes simplex virus type 1-based amplicon vectors.Nunez R, Ackermann M, Saeki Y, Chiocca A, Fraefel C.
Cytometry. 2001 Jun 1;44(2):93-9

Viren-Werkzeuge der Gentherapeuten.
Müller L., T. Heister and C. Fraefel
BioWorld 4:14-16 (2001). Review (on request)

HSV-1 infected cell proteins influence tetracycline-regulated transgene expression.
Herrlinger U, Pechan PA, Jacobs AH, Woiciechowski C, Rainov NG, Fraefel C, Paulus W, Reeves SA.
J Gene Med. 2000 Sep-Oct;2(5):379-89

Helper virus-free herpes simplex virus type 1 amplicon vectors for granulocyte-macrophage colony-stimulating factor-enhanced vaccination therapy for experimental glioma.
Herrlinger U, Jacobs A, Quinones A, Woiciechowsky C, Sena-Esteves M, Rainov NG, Fraefel C, Breakefield XO.
Hum Gene Ther. 2000 Jul 1;11(10):1429-38

HSV-1 amplicon vectors are a highly efficient gene delivery system for skeletal muscle myoblasts and myotubes.Wang Y, Fraefel C, Protasi F, Moore RA, Fessenden JD, Pessah IN, DiFrancesco A, Breakefield X, Allen PD.
Am J Physiol Cell Physiol. 2000 Mar;278(3):C619-26. doi: 10.1152/ajpcell.2000.278.3.C619.

Herpes simplex virus type 1-based amplicon vector systems
Fraefel C., D. Jacoby and X.O. Breakefield
Adv. Virus Res. 2000:55:425-451. doi: 10.1016/s0065-3527(00)55011-8

HSV-1 amplicon vectors-simplicity and versatility.
Sena-Esteves M., Y. Saeki, C. Fraefel and X.O. Breakefield
Mol. Ther.2000 Jul;2(1):9-15 doi: 10.1006/mthe.2000.0096

HSV-1 amplicon and HSV/AAV-based hybrid amplicon vectors for gene transfer experiments in cells in culture and in vivo.
Saeki Y. and C. Fraefel
In: Cid-Arregui A. and A. Garcia-Carranca (eds.), Viral vectors: Basic science and gene therapy. Eaton Publishing, Natick, MA; 211-233 (2000) / Book, on request

Gene transfer to the nigrostriatal system by hybrid herpes simplex virus/adeno-associated virus amplicon vectors.Costantini LC, Jacoby DR, Wang S, Fraefel C, Breakefield XO, Isacson O.
Hum Gene Ther. 1999 Oct 10;10(15):2481-94. Erratum in: Hum Gene Ther 2000 Apr 10;11(6):981

BAC-VAC, a novel generation of (DNA) vaccines: A bacterial artificial chromosome (BAC) containing a replication-competent, packaging-defective virus genome induces protective immunity against herpes simplex virus 1.Suter M, Lew AM, Grob P, Adema GJ, Ackermann M, Shortman K, Fraefel C.
Proc Natl Acad Sci U S A. 1999 Oct 26;96(22):12697-702

Mutant huntingtin forms in vivo complexes with distinct context-dependent conformations of the polyglutamine segment.Persichetti F, Trettel F, Huang CC, Fraefel C, Timmers HT, Gusella JF, MacDonald ME.
Neurobiol Dis. 1999 Oct;6(5):364-75

HSV-1-Based Vectors for Gene Therapy of Neurological Diseases and Brain Tumors: Part I. HSV-1 Structure, Replication and Pathogenesis.
Jacobs A., X.O. Breakefield and C. Fraefel
Neoplasia 1999 Nov; 1(5):387-401 doi: 10.1038/sj.neo.7900055

HSV-1-Based Vectors for Gene Therapy of Neurological Diseases and Brain Tumors: Part II.
Jacobs A., X.O. Breakefield and C. Fraefel
Vector Systems and Applications. Neoplasia 1999 Nov;1(5): 402-416 doi:10.1038/sj.neo.7900056

Gene delivery using helper virus-free HSV-1 amplicon vectors.
In: Crawley J., et al. (eds.), Current Protocols in Neurosciences.Fraefel C
John Wiley & Sons, Inc., New York; 4.14.1.-4.14.16 (1999), on request

Helper virus-free herpesvirus vector packaging system.
Fraefel C., A.I. Geller and F. Lim
(1998/1999). United States Patents 5,851,826 and 5,998,208

Herpes simplex virus type 1 DNA amplified as bacterial artificial chromosome in Escherichia coli: rescue of replication-competent virus progeny and packaging of amplicon vectors.Saeki Y, Ichikawa T, Saeki A, Chiocca EA, Tobler K, Ackermann M, Breakefield XO, Fraefel C.
Hum Gene Ther. 1998 Dec 10;9(18):2787-94

A chimeric fusion protein of cytochrome CYP4B1 and green fluorescent protein for detection of pro-drug activating gene delivery and for gene therapy in malignant glioma.
Rainov N.G., M. Sena-Esteves, C. Fraefel, K.U. Dobberstein, E.A. Chiocca and X.O. Breakefield
Adv. Exp. Med. Biol. 1998:451:393-403 doi: 10.1007/978-1-4615-5357-1_61

HSV-1 amplicon.
In: Chiocca E.A. and X.O. Breakefield (eds.), Gene therapy for neurological disorders and brain tumors.Fraefel C., X.O. Breakefield and D. Jacoby
Humana Press, Totowa; 63-82 (1998)

Gene therapy for ischemic stroke.
Pechan P., M. Fujii, C. Fraefel, A. Jacobs and M.A. Moskowitz
In: Chiocca E.A. and X.O. Breakefield (eds.), Gene therapy for neurological disorders and brain tumors. Humana Press, Totowa; 397-408 (1998).